Lack of evidence for a role of islet autoimmunity in the aetiology of canine diabetes mellitus.

ABSTRACT:

Aims/hypothesis

Diabetes mellitus is one of the most common endocrine disorders in dogs and is commonly proposed to be of autoimmune origin. Although the clinical presentation of human type 1 diabetes (T1D) and canine diabetes are similar, the aetiologies may differ. The aim of this study was to investigate if autoimmune aetiology resembling human T1D is as prevalent in dogs as previously reported.

Methods

Sera from 121 diabetic dogs representing 40 different breeds were tested for islet cell antibodies (ICA) and GAD65 autoantibodies (GADA) and compared with sera from 133 healthy dogs. ICA was detected by indirect immunofluorescence using both canine and human frozen sections. GADA was detected by in vitro transcription and translation (ITT) of human and canine GAD65, follow

SUBMITTER: Ahlgren KM 

PROVIDER: S-EPMC4143278 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature