Widespread changes in the posttranscriptional landscape at the Drosophila oocyte-to-embryo transition.

ABSTRACT: The oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling, and quantitative mass spectrometry in a comprehensive approach to delineate the translational and proteomic changes that occur during this important transition in Drosophila. Our results show that PNG kinase is a critical regulator of the extensive changes in the translatome, acting uniquely at this developmental window. Analysis of the proteome in png mutants provided insights into the contributions of translation to changes in protein levels, revealing a compensatory dynamic between translati

SUBMITTER: Kronja I 

PROVIDER: S-EPMC4143395 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature