The clustering of CpG islands may constitute an important determinant of the 3D organization of interphase chromosomes.

ABSTRACT: We used the 4C-Seq technique to characterize the genome-wide patterns of spatial contacts of several CpG islands located on chromosome 14 in cultured chicken lymphoid and erythroid cells. We observed a clear tendency for the spatial clustering of CpG islands present on the same and different chromosomes, regardless of the presence or absence of promoters within these CpG islands. Accordingly, we observed preferential spatial contacts between Sp1 binding motifs and other GC-rich genomic elements, including the DNA sequence motifs capable of forming G-quadruplexes. However, an anchor placed in a gene/CpG island-poor area formed spatial contacts with other gene/CpG island-poor areas on chromosome 14 and other chromosomes. These results corroborate the two-compartment model of the spatial orga

SUBMITTER: Gushchanskaya ES 

PROVIDER: S-EPMC4143410 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature