Validated gene expression biomarker analysis for biopsy-based clinical trials in ulcerative colitis.

ABSTRACT:

Background

Accurate and reproducible measurement of expression of pro-inflammatory cytokines in colonic biopsies from patients with ulcerative colitis (UC) is essential for proof-of-concept and mechanism-of-action studies. Few studies have rigorously established the number of biopsies required for accurate and reproducible biomarker measurements.

Aim

To validate methods for measuring changes in gene expression in colonic biopsy samples.

Methods

Twelve colonic biopsies were obtained from each of six healthy controls, six patients with inactive UC and seven patients with active UC. Mayo endoscopic scores were used as a clinical reference standard. Quantitative PCR was used to assess mRNA expression of eight known inflammatory genes. The power to detect a reduction in ge

SUBMITTER: Boland BS 

PROVIDER: S-EPMC4143528 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature