Evolution of substrate specificity in bacterial AA10 lytic polysaccharide monooxygenases.

ABSTRACT:

Background

Understanding the diversity of lignocellulose-degrading enzymes in nature will provide insights for the improvement of cellulolytic enzyme cocktails used in the biofuels industry. Two families of enzymes, fungal AA9 and bacterial AA10, have recently been characterized as crystalline cellulose or chitin-cleaving lytic polysaccharide monooxygenases (LPMOs). Here we analyze the sequences, structures, and evolution of LPMOs to understand the factors that may influence substrate specificity both within and between these enzyme families.

Results

Comparative analysis of sequences, solved structures, and homology models from AA9 and AA10 LPMO families demonstrated that, although these two LPMO families are highly conserved, structurally they have minimal sequence similari

SUBMITTER: Book AJ 

PROVIDER: S-EPMC4144037 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature