Molecular architecture of the Goodpasture autoantigen in anti-GBM nephritis.

ABSTRACT:

Background

In Goodpasture's disease, circulating autoantibodies bind to the noncollagenous-1 (NC1) domain of type IV collagen in the glomerular basement membrane (GBM). The specificity and molecular architecture of epitopes of tissue-bound autoantibodies are unknown. Alport's post-transplantation nephritis, which is mediated by alloantibodies against the GBM, occurs after kidney transplantation in some patients with Alport's syndrome. We compared the conformations of the antibody epitopes in Goodpasture's disease and Alport's post-transplantation nephritis with the intention of finding clues to the pathogenesis of anti-GBM glomerulonephritis.

Methods

We used an enzyme-linked immunosorbent assay to determine the specificity of circulating autoantibodies and kidney-bound antib

SUBMITTER: Pedchenko V 

PROVIDER: S-EPMC4144421 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature