Cutting edge: cell surface linker for activation of T cells is recruited to microclusters and is active in signaling.

ABSTRACT: A controversy has recently emerged regarding the location of the cellular pool of the adapter linker for activation of T cells (LAT) that participates in propagation of signals downstream of the TCR. In one model phosphorylation and direct recruitment of cell surface LAT to activation-induced microclusters is critical for T cell activation, whereas in the other model vesicular, but not surface, LAT participates in these processes. By using a chimeric version of LAT that can be tracked via an extracellular domain, we provide evidence that LAT located at the cell surface can be recruited efficiently to activation-induced microclusters within seconds of TCR engagement. Importantly, we also demonstrate that this pool of LAT at the plasma membrane is rapidly phosphorylated. Our results provide

SUBMITTER: Balagopalan L 

PROVIDER: S-EPMC4144449 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature