A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays.

ABSTRACT: We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that seen with a conventional CXCR4 antibody. This nonradioactive, direct, and CXCR4-specific high-affinity screening system provides a new platform for CXCR4-targeted drug screening, as well as for the development of new probes for other GPCRs.

SUBMITTER: Yang Y 

PROVIDER: S-EPMC4144712 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature