Gametocytocidal screen identifies novel chemical classes with Plasmodium falciparum transmission blocking activity.

ABSTRACT: Discovery of transmission blocking compounds is an important intervention strategy necessary to eliminate and eradicate malaria. To date only a small number of drugs that inhibit gametocyte development and thereby transmission from the mosquito to the human host exist. This limitation is largely due to a lack of screening assays easily adaptable to high throughput because of multiple incubation steps or the requirement for high gametocytemia. Here we report the discovery of new compounds with gametocytocidal activity using a simple and robust SYBR Green I- based DNA assay. Our assay utilizes the exflagellation step in male gametocytes and a background suppressor, which masks the staining of dead cells to achieve healthy signal to noise ratio by increasing signal of viable parasites and sub

SUBMITTER: Sanders NG 

PROVIDER: S-EPMC4144897 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature