DNA methylation dynamics during ex vivo differentiation and maturation of human dendritic cells.

ABSTRACT:

Background

Dendritic cells (DCs) are important mediators of innate and adaptive immune responses, but the gene networks governing their lineage differentiation and maturation are poorly understood. To gain insight into the mechanisms that promote human DC differentiation and contribute to the acquisition of their functional phenotypes, we performed genome-wide base-resolution mapping of 5-methylcytosine in purified monocytes and in monocyte-derived immature and mature DCs.

Results

DC development and maturation were associated with a great loss of DNA methylation across many regions, most of which occurs at predicted enhancers and binding sites for known transcription factors affiliated with DC lineage specification and response to immune stimuli. In addition, we discovered n

SUBMITTER: Zhang X 

PROVIDER: S-EPMC4144987 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature