A microchip platform for interrogating tumor-macrophage paracrine signaling at the single-cell level.

ABSTRACT: It is increasingly recognized that infiltrating immune cells contribute to the pathogenesis of a wide range of solid tumors. The paracrine signaling between the tumor and the immune cells alters the functional state of individual tumor cells and, correspondingly, the anticipated response to radiation or chemotherapies, which is of great importance to clinical oncology. Here we present a high-density microchip platform capable of measuring a panel of paracrine signals associated with heterotypic tumor-immune cell interactions in the single-cell, pair-wise manner. The device features a high-content cell capture array of 5000+ sub-nanoliter microchambers for the isolation of single and multi-cell combinations and a multi-plex antibody "barcode" array for multiplexed protein secretion analysis

SUBMITTER: Elitas M 

PROVIDER: S-EPMC4145007 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature