Rational and combinatorial approaches to engineering styrene production by Saccharomyces cerevisiae.

ABSTRACT:

Background

Styrene is an important building-block petrochemical and monomer used to produce numerous plastics. Whereas styrene bioproduction by Escherichia coli was previously reported, the long-term potential of this approach will ultimately rely on the use of hosts with improved industrial phenotypes, such as the yeast Saccharomyces cerevisiae.

Results

Classical metabolic evolution was first applied to isolate a mutant capable of phenylalanine over-production to 357 mg/L. Transcription analysis revealed up-regulation of several phenylalanine biosynthesis pathway genes including ARO3, encoding the bottleneck enzyme DAHP synthase. To catalyze the first pathway step, phenylalanine ammonia lyase encoded by PAL2 from A. thaliana was constitutively expressed from a high copy pla

SUBMITTER: McKenna R 

PROVIDER: S-EPMC4145238 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature