Rectal microRNAs are perturbed in pediatric inflammatory bowel disease of the colon.

ABSTRACT:

Background and aims

Changes in intestinal microRNAs have been reported in adult patients with ulcerative colitis or Crohn's disease. The goal of this study was to identify changes in microRNA expression associated with colitis in children with inflammatory bowel disease.

Methods

Rectal mucosal biopsies (n = 50) and blood samples (n = 47) were collected from patients with known or suspected inflammatory bowel disease undergoing endoscopy. Rectal and serum microRNA levels were profiled using the nCounter platform and the TaqMan low-density array platform, respectively. Significantly altered microRNAs were validated in independent sample sets via quantitative RT-PCR. In vitro luciferase reporter assays were performed in the human colorectal Caco-2 cell line to determine the eff

SUBMITTER: Zahm AM 

PROVIDER: S-EPMC4146627 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature