Mammalian target of rapamycin cell signaling pathway contributes to the protective effects of ischemic postconditioning against stroke.

ABSTRACT:

Background and purpose

Whether the mammalian target of rapamycin (mTOR) pathway is protective against brain injury from stroke or is detrimental is controversial, and whether it is involved in the protective effects of ischemic postconditioning (IPC) against stroke is unreported. Our study focuses on the protective role of mTOR against neuronal injury after stroke with and without IPC.

Methods

We used both an in vitro oxygen-glucose deprivation model with a mixed neuronal culture and hypoxic postconditioning, as well as an in vivo stroke model with IPC. Rapamycin, a specific pharmacological inhibitor of mTOR, and mTOR short hairpin RNA lentiviral vectors were used to inhibit mTOR activity. A lentiviral vector expressing S6K1, a downstream molecule of mTOR, was used to confir

SUBMITTER: Xie R 

PROVIDER: S-EPMC4146669 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature