Gene expression profile analyses of mice livers injured by Leigongteng.

ABSTRACT:

Aim

To analyze the gene expression profiles of mice livers injured by Leigongteng and explore the relationship between the differentially expressed genes and liver damage.

Methods

The experimental mice were randomly divided into a control group and a liver-injured group in which the mice were administrated 33 mu gamma of triptolide/kg per day for 30 d. Liver mRNAs were extracted from animals in both groups and were reverse-transcribed to cDNA with dUTP labeled by different fluorescence (Cy3, Cy5) as hybridization probes. The mixed probes were hybridized with oligonucleotide microarray chips. The fluorescent signal results were acquired by scanner and analyzed with software.

Results

Among the 35852 target genes, 29 genes were found to be significantly differentially ex

SUBMITTER: Chen Y 

PROVIDER: S-EPMC4146803 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature