Protein profile of human hepatocarcinoma cell line SMMC-7721: identification and functional analysis.

ABSTRACT:

Aim

To investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to explore the new drug targets for liver cancer therapy.

Methods

Total proteins from human hepatocarcinoma cell line SMMC-7721 were separated by two-dimensional electrophoresis (2DE). The silver-stained gel was analyzed by 2DE software Image Master 2D Elite. Interesting protein spots were identified by peptide mass fingerprinting based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.

Results

SUBMITTER: Feng Y 

PROVIDER: S-EPMC4146824 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature