Expression of p21(WAF1) is related to acetylation of histone H3 in total chromatin in human colorectal cancer.

ABSTRACT: AIM:To explore the relationship between acetylation of histone in total chromatin and p21(WAF1) expression regulation in human colorectal carcinoma. METHODS:We analyzed the expression of tumor suppressor gene p21(WAF1) mRNA by RT-PCR or real-time PCR in 33 samples of colorectal cancerous tissue, corresponding para-cancerous tissue and normal colorectal mucosa, and also examined the level of acetylated histone H3 in total chromatin using Western blotting. RESULTS:The expression level of p21(WAF1) mRNA was significantly lower in colorectal cancerous tissue from 33 patients than in para-cancerous tissue and normal colorectal mucosa (2377.95 +/- 865.80 vs 3216.58 +/- 1149.42 and 3541.61 +/- 1433.17 respectively, P < 0.01). In addition, when p21(WAF1) mRNA expression was undectectable or at ver

SUBMITTER: Chen YX 

PROVIDER: S-EPMC4146846 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature