Expression of SNC73, a transcript of the immunoglobulin alpha-1 gene, in human epithelial carcinomas.

ABSTRACT:

Aim

To investigate the expression of SNC73, a trans-cript of the immunoglobulin alpha-1 gene (IgA1-H chain), in human epithelia-derived tumor cells.

Methods

Total RNAs and cell lysates were prepared from five different human epithelial cell lines derived from lung, stomach, liver, skin, and breast, respectively. RT-PCR and immunoblot analysis of these five cell lines were done. Both RT-PCR and immunochemistry were used to detect the expression of SNC73 in these cell lines. We also examined the expression of SNC73 in normal epithelial cells of colon mucosa by in situ hybridization. RT-PCR and immunoblot analysis were used to determine whether the recombination activating gene1/2 (RAG1 and RAG2) is present. The expression of three immunoglobulin transcription factors, EBF, E2A

SUBMITTER: Geng LY 

PROVIDER: S-EPMC4147138 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature