DNA transport across the outer and inner membranes of naturally transformable Vibrio cholerae is spatially but not temporally coupled.

ABSTRACT: The physiological state of natural competence for transformation allows certain bacteria to take up free DNA from the environment and to recombine such newly acquired DNA into their chromosomes. However, even though conserved components that are required to undergo natural transformation have been identified in several naturally competent bacteria, our knowledge of the underlying mechanisms of the DNA uptake process remains very limited. To better understand these mechanisms, we investigated the competence-mediated DNA transport in the naturally transformable pathogen Vibrio cholerae. Previously, we used a cell biology-based approach to experimentally address an existing hypothesis, which suggested the competence protein ComEA plays a role in the DNA uptake process across the outer membran

SUBMITTER: Seitz P 

PROVIDER: S-EPMC4147865 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature