MethylSig: a whole genome DNA methylation analysis pipeline.

ABSTRACT:

Motivation

DNA methylation plays critical roles in gene regulation and cellular specification without altering DNA sequences. The wide application of reduced representation bisulfite sequencing (RRBS) and whole genome bisulfite sequencing (bis-seq) opens the door to study DNA methylation at single CpG site resolution. One challenging question is how best to test for significant methylation differences between groups of biological samples in order to minimize false positive findings.

Results

We present a statistical analysis package, methylSig, to analyse genome-wide methylation differences between samples from different treatments or disease groups. MethylSig takes into account both read coverage and biological variation by utilizing a beta-binomial approach across biologica

SUBMITTER: Park Y 

PROVIDER: S-EPMC4147891 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature