Microarray R-based analysis of complex lysate experiments with MIRACLE.

ABSTRACT:

Motivation

Reverse-phase protein arrays (RPPAs) allow sensitive quantification of relative protein abundance in thousands of samples in parallel. Typical challenges involved in this technology are antibody selection, sample preparation and optimization of staining conditions. The issue of combining effective sample management and data analysis, however, has been widely neglected.

Results

This motivated us to develop MIRACLE, a comprehensive and user-friendly web application bridging the gap between spotting and array analysis by conveniently keeping track of sample information. Data processing includes correction of staining bias, estimation of protein concentration from response curves, normalization for total protein amount per sample and statistical evaluation. Establishe

SUBMITTER: List M 

PROVIDER: S-EPMC4147925 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature