Fluorescence microangiography for quantitative assessment of peritubular capillary changes after AKI in mice.

ABSTRACT: AKI predicts the future development of CKD, and one proposed mechanism for this epidemiologic link is loss of peritubular capillaries triggering chronic hypoxia. A precise definition of changes in peritubular perfusion would help test this hypothesis by more accurately correlating these changes with future loss of kidney function. Here, we have adapted and validated a fluorescence microangiography approach for use with mice to visualize, analyze, and quantitate peritubular capillary dynamics after AKI. A novel software-based approach enabled rapid and automated quantitation of capillary number, individual area, and perimeter. After validating perfusion in mice with genetically labeled endothelia, we compared peritubular capillary number and size after moderate AKI, characterized by complet

SUBMITTER: Kramann R 

PROVIDER: S-EPMC4147987 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature