Differential gene expression profile of first-generation and second-generation rapamycin-resistant allogeneic T cells.

ABSTRACT:

Background aims

We completed a phase II clinical trial evaluating rapamycin-resistant allogeneic T cells (T-rapa) and now have evaluated a T-rapa product manufactured in 6 days (T-rapa(6)) rather than 12 days (T-Rapa(12)).

Methods

Using gene expression microarrays, we addressed our hypothesis that the two products would express a similar phenotype. The products had similar phenotypes using conventional comparison methods of cytokine secretion and surface markers.

Results

Unsupervised analysis of 34,340 genes revealed that T-rapa(6) and T-rapa(12) products clustered together, distinct from culture input CD4(+) T cells. Statistical analysis of T-rapa(6) products revealed differential expression of 19.3% of genes (n = 6641) compared with input CD4(+) cells; similarly, 17

SUBMITTER: Castiello L 

PROVIDER: S-EPMC4148082 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature