Protein surface softness is the origin of enzyme cold-adaptation of trypsin.

ABSTRACT: Life has effectively colonized most of our planet and extremophilic organisms require specialized enzymes to survive under harsh conditions. Cold-loving organisms (psychrophiles) express heat-labile enzymes that possess a high specific activity and catalytic efficiency at low temperatures. A remarkable universal characteristic of cold-active enzymes is that they show a reduction both in activation enthalpy and entropy, compared to mesophilic orthologs, which makes their reaction rates less sensitive to falling temperature. Despite significant efforts since the early 1970s, the important question of the origin of this effect still largely remains unanswered. Here we use cold- and warm-active trypsins as model systems to investigate the temperature dependence of the reaction rates with exten

SUBMITTER: Isaksen GV 

PROVIDER: S-EPMC4148182 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature