Chromatin insulator factors involved in long-range DNA interactions and their role in the folding of the Drosophila genome.

ABSTRACT: Chromatin insulators are genetic elements implicated in the organization of chromatin and the regulation of transcription. In Drosophila, different insulator types were characterized by their locus-specific composition of insulator proteins and co-factors. Insulators mediate specific long-range DNA contacts required for the three dimensional organization of the interphase nucleus and for transcription regulation, but the mechanisms underlying the formation of these contacts is currently unknown. Here, we investigate the molecular associations between different components of insulator complexes (BEAF32, CP190 and Chromator) by biochemical and biophysical means, and develop a novel single-molecule assay to determine what factors are necessary and essential for the formation of long-range DNA

SUBMITTER: Vogelmann J 

PROVIDER: S-EPMC4148193 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature