Optimized multilocus sequence typing (MLST) scheme for Trypanosoma cruzi.

ABSTRACT: Trypanosoma cruzi, the aetiological agent of Chagas disease possess extensive genetic diversity. This has led to the development of a plethora of molecular typing methods for the identification of both the known major genetic lineages and for more fine scale characterization of different multilocus genotypes within these major lineages. Whole genome sequencing applied to large sample sizes is not currently viable and multilocus enzyme electrophoresis, the previous gold standard for T. cruzi typing, is laborious and time consuming. In the present work, we present an optimized Multilocus Sequence Typing (MLST) scheme, based on the combined analysis of two recently proposed MLST approaches. Here, thirteen concatenated gene fragments were applied to a panel of T. cruzi reference strains encomp

SUBMITTER: Diosque P 

PROVIDER: S-EPMC4148231 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature