Cross talk with hematopoietic cells regulates the endothelial progenitor cell differentiation of CD34 positive cells.

ABSTRACT:

Introduction

Despite the crucial role of endothelial progenitor cells (EPCs) in vascular regeneration, the specific interactions between EPCs and hematopoietic cells remain unclear.

Methods

In EPC colony forming assays, we first demonstrated that the formation of EPC colonies was drastically increased in the coculture of CD34+ and CD34- cells, and determined the optimal concentrations of CD34+ cells and CD34- cells for spindle-shaped EPC differentiation.

Results

Functionally, the coculture of CD34+ and CD34- cells resulted in a significant enhancement of adhesion, tube formation, and migration capacity compared with culture of CD34+ cells alone. Furthermore, blood flow recovery and capillary formation were remarkably increased by the coculture of CD34+ and CD34- cells

SUBMITTER: Kwon SM 

PROVIDER: S-EPMC4148437 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature