Human cytomegalovirus pUL79 is an elongation factor of RNA polymerase II for viral gene transcription.

ABSTRACT: In this study, we have identified a unique mechanism in which human cytomegalovirus (HCMV) protein pUL79 acts as an elongation factor to direct cellular RNA polymerase II for viral transcription during late times of infection. We and others previously reported that pUL79 and its homologues are required for viral transcript accumulation after viral DNA synthesis. We hypothesized that pUL79 represented a unique mechanism to regulate viral transcription at late times during HCMV infection. To test this hypothesis, we analyzed the proteome associated with pUL79 during virus infection by mass spectrometry. We identified both cellular transcriptional factors, including multiple RNA polymerase II (RNAP II) subunits, and novel viral transactivators, including pUL87 and pUL95, as protein binding pa

SUBMITTER: Perng YC 

PROVIDER: S-EPMC4148446 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature