A cellular genetics approach identifies gene-drug interactions and pinpoints drug toxicity pathway nodes.

ABSTRACT: New approaches to toxicity testing have incorporated high-throughput screening across a broad-range of in vitro assays to identify potential key events in response to chemical or drug treatment. To date, these approaches have primarily utilized repurposed drug discovery assays. In this study, we describe an approach that combines in vitro screening with genetic approaches for the experimental identification of genes and pathways involved in chemical or drug toxicity. Primary embryonic fibroblasts isolated from 32 genetically-characterized inbred mouse strains were treated in concentration-response format with 65 compounds, including pharmaceutical drugs, environmental chemicals, and compounds with known modes-of-action. Integrated cellular responses were measured at 24 and 72 h using high-

SUBMITTER: Suzuki OT 

PROVIDER: S-EPMC4148776 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature