Novel outer membrane protein involved in cellulose and cellooligosaccharide degradation by Cytophaga hutchinsonii.

ABSTRACT: Cytophaga hutchinsonii is an aerobic cellulolytic soil bacterium which was reported to use a novel contact-dependent strategy to degrade cellulose. It was speculated that cellooligosaccharides were transported into the periplasm for further digestion. In this study, we reported that most of the endoglucanase and -glucosidase activity was distributed on the cell surface of C. hutchinsonii.Cellobiose and part of the cellulose could be hydrolyzed to glucose on the cell surface. However, the cell surface cellulolytic enzymes were not sufficient for cellulose degradation by C. hutchinsonii. An outer membrane protein, CHU_1277, was disrupted by insertional mutation. Although the mutant maintained the same endoglucanase activity and most of the -glucosidase activity,it failed to digest cellulose,

SUBMITTER: Ji X 

PROVIDER: S-EPMC4148786 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature