Metabolic shift of Escherichia coli under salt stress in the presence of glycine betaine.

ABSTRACT: An important area of food safety focuses on bacterial survival and growth in unfavorable environments. In order to understand how bacteria adapt to stresses other than nutrient limitation in batch cultures, we need to develop mechanistic models of intracellular regulation and metabolism under stress. We studied the growth of Escherichia coli in minimal medium with added salt and different osmoprotectants. To characterize the metabolic efficiency with a robust parameter, we identified the optical density (OD) values at the inflection points of measured "OD versus time" growth curves and described them as a function of glucose concentration. We found that the metabolic efficiency parameter did not necessarily follow the trend of decreasing specific growth rate as the salt concentration incre

SUBMITTER: Metris A 

PROVIDER: S-EPMC4148810 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature