Peptide-dependent conformational fluctuation determines the stability of the human leukocyte antigen class I complex.

ABSTRACT: In immune-mediated control of pathogens, human leukocyte antigen (HLA) class I presents various antigenic peptides to CD8(+) T-cells. Long-lived peptide presentation is important for efficient antigen-specific T-cell activation. Presentation time depends on the peptide sequence and the stability of the peptide-HLA complex (pHLA). However, the determinant of peptide-dependent pHLA stability remains elusive. Here, to reveal the pHLA stabilization mechanism, we examined the crystal structures of an HLA class I allomorph in complex with HIV-derived peptides and evaluated site-specific conformational fluctuations using NMR. Although the crystal structures of various pHLAs were almost identical independent of the peptides, fluctuation analyses identified a peptide-dependent minor state that woul

SUBMITTER: Yanaka S 

PROVIDER: S-EPMC4148890 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature