Hypoxic signature of microRNAs in glioblastoma: insights from small RNA deep sequencing.

ABSTRACT:

Background

Hypoxia is a critical aspect of the glioma microenvironment and has been associated with poor prognosis and resistance to various therapies. However, the mechanisms responsible for hypoxic survival of glioma cells remain unclear. Recent studies strongly suggest that microRNAs act as critical mediators of the hypoxic response. We thus hypothesized their prominent role in hypoxia resistance in glioblastoma (GBM) and aimed to identify those.

Results

With this study, we present the first detailed analysis of small RNA transcriptome of cell line U87MG, a grade IV glioma cell line, and its alteration under hypoxic condition. Based on deep sequencing and microarray data, we identify a set of hypoxia regulated microRNAs, with the miR-210-3p and its isomiRs showing highest

SUBMITTER: Agrawal R 

PROVIDER: S-EPMC4148931 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature