Denitrification in agriculturally impacted streams: seasonal changes in structure and function of the bacterial community.

ABSTRACT: Denitrifiers remove fixed nitrogen from aquatic environments and hydrologic conditions are one potential driver of denitrification rate and denitrifier community composition. In this study, two agriculturally impacted streams in the Sugar Creek watershed in Indiana, USA with different hydrologic regimes were examined; one stream is seasonally ephemeral because of its source (tile drainage), whereas the other stream has permanent flow. Additionally, a simulated flooding experiment was performed on the riparian benches of the ephemeral stream during a dry period. Denitrification activity was assayed using the chloramphenicol amended acetylene block method and bacterial communities were examined based on quantitative PCR and terminal restriction length polymorphisms of the nitrous oxide reduc

SUBMITTER: Manis E 

PROVIDER: S-EPMC4149370 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature