Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli.

ABSTRACT: A key role for podocytes in the pathogenesis of proteinuric renal diseases has been established. Angiotensin II causes depolarization and increased intracellular calcium concentration in podocytes; members of the cation TRPC channels family, particularly TRPC6, are proposed as proteins responsible for calcium flux. Angiotensin II evokes calcium transient through TRPC channels and mutations in the gene encoding the TRPC6 channel result in the development of focal segmental glomerulosclerosis. Here we examined the effects of angiotensin II on intracellular calcium ion levels and endogenous channels in intact podocytes of freshly isolated decapsulated mouse glomeruli. An ion channel with distinct TRPC6 properties was identified in wild-type, but was absent in TRPC6 knockout mice. Single-chann

SUBMITTER: Ilatovskaya DV 

PROVIDER: S-EPMC4149864 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature