Enhanced innate type 2 immune response in peripheral blood from patients with asthma.

ABSTRACT:

Background

In mice, group 2 innate lymphoid cells (ILC2s) likely mediate helminth immunity, inflammation, and tissue repair and remodeling. However, the involvement of ILC2s in human diseases, such as asthma, is not well understood.

Objectives

The goals of this study were to investigate whether peripheral blood specimens can be used to monitor innate type 2 immunity in human subjects and to examine whether ILC2s are involved in human asthma.

Methods

PBMCs from subjects with allergic asthma (AA), subjects with allergic rhinitis (AR), or healthy control (HC) subjects were cultured in vitro with IL-25 or IL-33. Flow cytometry and cell sorting were used to identify, isolate, and quantitate ILC2s in PBMCs.

Results

Human PBMCs produced IL-5 and IL-13 when stimulated

SUBMITTER: Bartemes KR 

PROVIDER: S-EPMC4149890 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature