Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components.

ABSTRACT:

Background

Airway remodeling might explain lung function decline among asthmatic children. Extracellular matrix (ECM) deposition by human lung fibroblasts (HLFs) is implicated in airway remodeling. Airway epithelial cell (AEC) signaling might regulate HLF ECM expression.

Objectives

We sought to determine whether AECs from asthmatic children differentially regulate HLF expression of ECM constituents.

Methods

Primary AECs were obtained from well-characterized atopic asthmatic (n = 10) and healthy (n = 10) children intubated during anesthesia for an elective surgical procedure. AECs were differentiated at an air-liquid interface for 3 weeks and then cocultured with HLFs from a healthy child for 96 hours. Collagen I (COL1A1), collagen III (COL3A1), hyaluronan synthase (HA

SUBMITTER: Reeves SR 

PROVIDER: S-EPMC4149938 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature