Cloning and expression of special F protein from human liver.

ABSTRACT:

Aim

To clone human liver special F protein and to express it in a prokaryotic system.

Methods

Total RNA was isolated from human liver tissue and first-strand cDNA was reverse transcribed using the PCR reverse primer. Following this, cDNA of the F protein was ligated into the clone vector pUCm-T. The segment of F protein's cDNA was subcloned into the expression vector pET-15b and transformed into E. coli BL21 (DE3) pLyss. Isopropy-beta-D-thiogalactoside (IPTG) was then used to induce expression of the target protein.

Results

The cDNA clone of human liver special F protein (1134bp) was successfully produced, with the cDNA sequence being published in Gene-bank: DQ188836. We confirmed the expression of F protein by Western blot with a molecular weight of 43 kDa. The expre

SUBMITTER: Liu SY 

PROVIDER: S-EPMC4149955 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature