Global absolute quantification reveals tight regulation of protein expression in single Xenopus eggs.

ABSTRACT: While recent developments in genomic sequencing technology have enabled comprehensive transcriptome analyses of single cells, single cell proteomics has thus far been restricted to targeted studies. Here, we perform global absolute protein quantification of fertilized Xenopus laevis eggs using mass spectrometry-based proteomics, quantifying over 5800 proteins in the largest single cell proteome characterized to date. Absolute protein amounts in single eggs are highly consistent, thus indicating a tight regulation of global protein abundance. Protein copy numbers in single eggs range from tens of thousands to ten trillion copies per cell. Comparison between the single-cell proteome and transcriptome reveal poor expression correlation. Finally, we identify 439 proteins that significantly cha

SUBMITTER: Smits AH 

PROVIDER: S-EPMC4150773 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature