Human papillomavirus type 16 long control region and E6 variants stratified by cervical disease stage.

ABSTRACT:

Objective

Certain intra-type variants of HPV16 have been shown to be associated with an increased risk of developing high grade cervical disease, but their potential association is confounded by apparent geographic and phylogenetic lineage dependency. The objective of this study was to evaluate the relationship between HPV16 sequence variants and cervical disease stage in monospecific infection samples from a single lineage (European, EUR) in England.

Methods

One hundred and twelve women singly infected with HPV16 and displaying normal and abnormal cytology grades were selected. An 1187 bp fragment encompassing the entire LCR and a portion of the E6 open reading frame was sequenced to identify intra-type variants. Intra-type diversity was estimated using Shannon entropy.

SUBMITTER: Marongiu L 

PROVIDER: S-EPMC4150919 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature