Depolymerase improves gentamicin efficacy during Klebsiella pneumoniae induced murine infection.

ABSTRACT:

Background

Presence of capsule enhances the virulence of bacteria that cause pneumonia, meningitis, cystic fibrosis, dental caries, periodontitis. Capsule is an important virulence factor for Klebsiella pneumoniae and infections due to this pathogen have been associated with high mortality rates. In the present study, use of an Aeromonas punctata derived capsule depolymerase against K. pneumoniae, to reinstate the efficacy of gentamicin during pneumonia and septicemia was investigated.

Methods

Depolymerase was administered in mice intraperitoneally (50 μg) alone as well in combination with gentamicin (1.5 mg/kg), 24 h post infection during acute lung infection and 6 h later during septicemia. Bacterial load, neutrophil infiltration and cytokine levels were estimated. The imm

SUBMITTER: Bansal S 

PROVIDER: S-EPMC4150946 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature