Proteomic analysis of the Plasmodium male gamete reveals the key role for glycolysis in flagellar motility.

ABSTRACT:

Background

Gametogenesis and fertilization play crucial roles in malaria transmission. While male gametes are thought to be amongst the simplest eukaryotic cells and are proven targets of transmission blocking immunity, little is known about their molecular organization. For example, the pathway of energy metabolism that power motility, a feature that facilitates gamete encounter and fertilization, is unknown.

Methods

Plasmodium berghei microgametes were purified and analysed by whole-cell proteomic analysis for the first time. Data are available via ProteomeXchange with identifier PXD001163.

Results

615 proteins were recovered, they included all male gamete proteins described thus far. Amongst them were the 11 enzymes of the glycolytic pathway. The hexose transporter

SUBMITTER: Talman AM 

PROVIDER: S-EPMC4150949 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature