Non-equilibrium folding of individual DNA molecules recaptured up to 1000 times in a solid state nanopore.

ABSTRACT: We investigate translocation of linear and circular double-stranded DNA molecules through solid state nanopores where each molecule is recaptured and re-translocated many times. Single molecules can be recaptured by switching voltage polarity for hundreds or even thousands of times. The large number of recapture events allows statistics on the translocation of individual molecules. Surprisingly, we observe that recaptured DNA molecules do not translocate in a linear head-to-tail fashion, but instead translocate as a folded blob where multiple parts of the DNA molecule simultaneously translocate through the pore in parallel. This folding is observed through the presence of up to 13 DNA double strands from the same molecule simultaneously inside the pore, as well as many smaller fold numbers

SUBMITTER: Plesa C 

PROVIDER: S-EPMC4151287 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature