High density lipoprotein is targeted for oxidation by myeloperoxidase in rheumatoid arthritis.

ABSTRACT:

Objective

Phagocyte-derived myeloperoxidase (MPO) and pro-inflammatory high density lipoprotein (HDL) associate with rheumatoid arthritis (RA), but the link between MPO and HDL has not been systematically examined. In this study, we investigated whether MPO can oxidise HDL and determined MPO-specific oxidative signature by apoA-1 by peptide mapping in RA subjects with and without known cardiovascular disease (CVD).

Methods

Two MPO oxidation products, 3-chlorotyrosine and 3-nitrotyrosine, were quantified by tandem mass spectrometry (MS/MS) in in vitro model system studies and in plasma and HDL derived from healthy controls and RA subjects. MPO levels and cholesterol efflux were determined. Site-specific nitration and chlorination of apoA-1 peptides were quantified by MS/MS.

SUBMITTER: Vivekanandan-Giri A 

PROVIDER: S-EPMC4151549 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature