A pretargeted PET imaging strategy based on bioorthogonal Diels-Alder click chemistry.

ABSTRACT:

Unlabelled

The specificity of antibodies have made immunoconjugates promising vectors for the delivery of radioisotopes to cancer cells; however, their long pharmacologic half-lives necessitate the use of radioisotopes with long physical half-lives, a combination that leads to high radiation doses to patients. Therefore, the development of targeting modalities that harness the advantages of antibodies without their pharmacokinetic limitations is desirable. To this end, we report the development of a methodology for pretargeted PET imaging based on the bioorthogonal Diels-Alder click reaction between tetrazine and transcyclooctene.

Methods

A proof-of-concept system based on the A33 antibody, SW1222 colorectal cancer cells, and (64)Cu was used. The huA33 antibody was covalentl

SUBMITTER: Zeglis BM 

PROVIDER: S-EPMC4151562 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature