MRI-localized biopsies reveal subtype-specific differences in molecular and cellular composition at the margins of glioblastoma.

ABSTRACT: Glioblastomas (GBMs) diffusely infiltrate the brain, making complete removal by surgical resection impossible. The mixture of neoplastic and nonneoplastic cells that remain after surgery form the biological context for adjuvant therapeutic intervention and recurrence. We performed RNA-sequencing (RNA-seq) and histological analysis on radiographically guided biopsies taken from different regions of GBM and showed that the tissue contained within the contrast-enhancing (CE) core of tumors have different cellular and molecular compositions compared with tissue from the nonenhancing (NE) margins of tumors. Comparisons with the The Cancer Genome Atlas dataset showed that the samples from CE regions resembled the proneural, classical, or mesenchymal subtypes of GBM, whereas the samples from the

SUBMITTER: Gill BJ 

PROVIDER: S-EPMC4151734 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature