Engineered kinase activation reveals unique morphodynamic phenotypes and associated trafficking for Src family isoforms.

ABSTRACT: The Src kinase family comprises nine homologous members whose distinct expression patterns and cellular distributions indicate that they have unique roles. These roles have not been determined because genetic manipulation has not produced clearly distinct phenotypes, and the kinases' homology complicates generation of specific inhibitors. Through insertion of a modified FK506 binding protein (insertable FKBP12, iFKBP) into the protein kinase isoforms Fyn, Src, Lyn, and Yes, we engineered kinase analogs that can be activated within minutes in living cells (RapR analogs). Combining our RapR analogs with computational tools for quantifying and characterizing cellular dynamics, we demonstrate that Src family isoforms produce very different phenotypes, encompassing cell spreading, polarized mot

SUBMITTER: Chu PH 

PROVIDER: S-EPMC4151743 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature