Identification of paralogous life-cycle stage specific cytoskeletal proteins in the parasite Trypanosoma brucei.

ABSTRACT: The life cycle of the African trypanosome Trypanosoma brucei, is characterised by a transition between insect and mammalian hosts representing very different environments that present the parasite with very different challenges. These challenges are met by the expression of life-cycle stage-specific cohorts of proteins, which function in systems such as metabolism and immune evasion. These life-cycle transitions are also accompanied by morphological rearrangements orchestrated by microtubule dynamics and associated proteins of the subpellicular microtubule array. Here we employed a gel-based comparative proteomic technique, Difference Gel Electrophoresis, to identify cytoskeletal proteins that are expressed differentially in mammalian infective and insect form trypanosomes. From this analy

SUBMITTER: Portman N 

PROVIDER: S-EPMC4152294 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature