Recombination between clonal lineages of the asexual fungus Verticillium dahliae detected by genotyping by sequencing.

ABSTRACT: Most asexual species of fungi have either lost sexuality recently, or they experience recombination by cryptic sexual reproduction. Verticillium dahliae is a plant-pathogenic, ascomycete fungus with no known sexual stage, even though related genera have well-described sexual reproduction. V. dahliae reproduces mitotically and its population structure is highly clonal. However, previously described discrepancies in phylogenetic relationships among clonal lineages may be explained more parsimoniously by recombination than mutation; therefore, we looked for evidence of recombination within and between clonal lineages. Genotyping by sequencing was performed on 141 V. dahliae isolates from diverse geographic and host origins, resulting in 26,748 single-nucleotide polymorphisms (SNPs). We found

SUBMITTER: Milgroom MG 

PROVIDER: S-EPMC4152335 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature