Autocrine signaling via release of ATP and activation of P2X7 receptor influences motile activity of human lung cancer cells.

ABSTRACT: Extracellular nucleotides, such as ATP, are released from cells and play roles in various physiological and pathological processes through activation of P2 receptors. Here, we show that autocrine signaling through release of ATP and activation of P2X7 receptor influences migration of human lung cancer cells. Release of ATP was induced by stimulation with TGF-β1, which is a potent inducer of cell migration, in human lung cancer H292 cells, but not in noncancerous BEAS-2B cells. Treatment of H292 cells with a specific antagonist of P2X7 receptor resulted in suppression of TGF-β1-induced migration. PC-9 human lung cancer cells released a large amount of ATP under standard cell culture conditions, and P2X7 receptor-dependent dye uptake was observed even in the absence of exogenous ligand, sugg

SUBMITTER: Takai E 

PROVIDER: S-EPMC4152450 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature